Utility of One Step Immunoassay in Detecting False Negativity in Routine Blood Bank Screening of Infectious Diseases
نویسنده
چکیده
Immunoassays are chemical tests used to detect or quantify a specific substance, the analyte, in a blood or body fluid sample, using an immunological reaction. Immunoassays for antibodies produced in viral hepatitis and HIV are commonly used to identify patients with these diseases.(Bishop et al., 2001). The commonly used immunoassay methods for detection of infectious diseases are immunoprecipitation, which measures the quantity of precipitate formed after the reagent antibody (precipitin) has been incubated with the sample and reacted with its respective antigen to form an insoluble aggregate, and enzyme immunoassay in the form of enzyme-linked immunosorbent assay (ELISA). The basic principle of these assays is the specificity of the antibody-antigen reaction.(Burtis & Ashwood,2001).
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